Ceramics-Silikáty 57, (3) 201 - 209 (2013) |
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SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF SOL–GEL DERIVED NANOMATERIAL IN THE TERNARY SYSTEM 64 % SiO2 - 31 % CaO - 5 % P2O5 AS A BIOACTIVE GLASS: IN VITRO STUDY
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Bizari D. 1, Rabiee M. 1, Moztarzadeh F. 1,
Tahriri M. 2,1,
Alavi S. H. 3,
Masaeli R. 2
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1 Biomaterial Group, Faculty of Biomedical Engineering (Center of Excellence),
Amirkabir University of Technology, P. O. Box: 15875-4413, Tehran, Iran
2 Dental Materials Department, School of Dentistry, Tehran University of Medical Sciences (TUMS), Tehran, Iran
3 School of Mechanical and Aerospace Engineering, Oklahoma State University, Stillwater, OK 74078, USA
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Keywords: Bioactive glass, Sol gel, In vitro, SBF, Osteoblastic cells
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In this study, we performed a new bioactive glass formulation with the molar composition 64 % SiO2 - 31 % CaO - 5 %
P2O5 by the sol-gel method. Sol-gel derived bioglass material was produced in nanopowder using planetary milling machine,
followed by sintering at 700oC, for applications as bioactive material in bioactive scaffolds or in orthopaedic. The obtained
material was evaluated by X-ray powder diffraction (XRD), thermal gravimetric analysis (TGA), differential scanning calorimetry
(DSC) analyses, Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and nitrogen
adsorption pore size. The biocompatibility evaluation of the formed glass was assessed through in vitro cell culture by
evaluation of alkaline phosphatase activity of osteoblasts and immersion studies in simulated body fluid (SBF) for different
time intervals while monitoring the pH changes and the concentration of calcium, phosphorus and silicon in the SBF medium
as key factors in the rapid bonding of this bioactive glass to bone tissue as a high bioactive glass. The present investigation
revealed that the sol-gel derived ternary bioglass system has the ability to support the growth of human fetal osteoblastic
cells (hFOB 1.19). Finally, this material proved to be non-toxic and compatible for the proposed work in segmental defects
in the goat model in vivo. |
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