Ceramics-Silikáty 52, (3) 121 - 129 (2008)

In our previous study, glass in weight ratio of 47% SiO₂ - 26% CaO - 21% Na₂O and 6% P₂O₅ have been prepared by the conventional melt quenching process. The referred glass have been characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy dispersive spectroscopy (EDS) confirming the composition and its amorphous state well. The in vitro bioactivity study of all pellets prepared were carried out by soaking in a simulated body fluid (SBF) for 1, 3, 7, 15, 20 and 30 days at 37°C. The analysis of SBF after each immersion time was carried out by inductively coupled plasma optical emission spectrometry (ICP/OES). The XRD, FTIR, SEM and EDS analysis of the glass surfaces after the in vitro essays, reveal the formation of an amorphous CaO–P₂O₅ - rich layer on the surface of the specimens after 20 days in the solution. After 30 days immersion time, a crystalline hydroxycarbonate apatite layer was formed that is similar in composition and crystallinity to the biological apatite. Osteoblast cell culture experiments were performed to assess the biocompatibility activity. Cell counts of the osteoblasts cultured on the bioactive glass samples were studied and compared with the polystyrene plates and Bioglass . The cells cultured on the bioactive 47S consistently highlighted the biocompatibility of this glass compared to cells cultured on polystyrene plates used as reference compounds.